Review




Structured Review

Promega goscript reverse transcriptase
Goscript Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscript+transcriptase/goscripttm+reverse+transcription+system/pmc12269245-32-8-16
Average 90 stars, based on 1 article reviews
goscript reverse transcriptase - by Bioz Stars, 2026-11
90/100 stars

Images

Related Articles

Quantitative RT-PCR:

Article Title: Reg4 + deep crypt secretory cells function as epithelial niche for Lgr5 + stem cells in colon
Article Snippet: Organoids were harvested in Buffer RLT lysis buffer after washing cold PBS, and total RNA was isolated by using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s protocol and treated with deoxyribonulease I (Qiagen). .. Mixture of random hexamers and oligo (dT) primers and GoScript transcriptase (Promega) were used for cDNA production. qRT-PCR was performed by using IQ-SYBR green mix (Bio-Rad) according to the manufacturer’s instructions. .. Three-dimensional representation was created by using Volocity (Improvision Ltd.).

Article Title: Inhibitors of TLR signaling by targeting TIR domain interfaces
Article Snippet: Protein was quantified using Bio-Rad protein quantification kit. .. Quantitative Real-Time RT-PCR Total RNA was isolated with Nucleospin RNA II kits (Macherey-Nagel, Bethlehem, Pa.), followed by DNase digestion. cDNA was synthesized from 1 μg RNA using Goscript transcriptase (Promega), and subjected to real-time PCR with gene-specific primers for HPRT, TNF-α, IL-1β, IFNβ, and RANTES on H7900 ABI system using Fast SYBR Green master mix (Applied Biosystems, Foster City, Calif.). .. The cell extracts containing equal amount of protein were incubated with 1 μg Ab for 2 h, followed by 4-h incubation with 25 μl protein G agarose beads (Roche); the beads were then washed four times with lysis buffer, boiled in 13 sample loading buffer, and analyzed by immunoblotting.

other:

Article Title: Could the kinetin riboside be used to inhibit human prostate cell epithelial-mesenchymal transition?
Article Snippet: Total amount of RNA was isolated using RNeasy Plus Mini kit (Qiagen, Hilden, Germany) according to manufacturer’s instruction.

Article Title: Inhibitors of TLR signaling by targeting TIR domain interfaces
Article Snippet: GFP Ab (A1122) was from Invitrogen.

Article Title: Differential adapter recruitment by TLR2 co-receptors.
Article Snippet: A total of 5 × 104 mouse peritoneal macrophages were plated into 96-well tissue culture plates, incubated overnight, and treated with peptides with or without TLR2 agonist for 2 or 5 h, followed by 3 h incubation with 0.5 mg ml−1 MTT (3- (4, 5-Dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) (Sigma-Aldrich).

Article Title: Differential adapter recruitment by TLR2 co-receptors
Article Snippet: Supernatants were spotted into PVDF membrane, followed by immunoblotting with rabbit anti-Antp Ab (Abcam, Cambridge, MA).

Isolation:

Article Title: Inhibitors of TLR signaling by targeting TIR domain interfaces
Article Snippet: Protein was quantified using Bio-Rad protein quantification kit. .. Quantitative Real-Time RT-PCR Total RNA was isolated with Nucleospin RNA II kits (Macherey-Nagel, Bethlehem, Pa.), followed by DNase digestion. cDNA was synthesized from 1 μg RNA using Goscript transcriptase (Promega), and subjected to real-time PCR with gene-specific primers for HPRT, TNF-α, IL-1β, IFNβ, and RANTES on H7900 ABI system using Fast SYBR Green master mix (Applied Biosystems, Foster City, Calif.). .. The cell extracts containing equal amount of protein were incubated with 1 μg Ab for 2 h, followed by 4-h incubation with 25 μl protein G agarose beads (Roche); the beads were then washed four times with lysis buffer, boiled in 13 sample loading buffer, and analyzed by immunoblotting.

Article Title: Differential adapter recruitment by TLR2 co-receptors
Article Snippet: Supernatants were spotted into PVDF membrane, followed by immunoblotting with rabbit anti-Antp Ab (Abcam, Cambridge, MA). .. cDNA was synthesized from 1 μg of total RNA isolated with Nucleospin RNA II kits (Macherey-Nagel, Inc., Bethlehem, PA) using Goscript transcriptase (Promega, Madison, WI), and subjected to real-time PCR (RT-PCR) with gene-specific primers for HPRT, TNF-α or IL-6 (Couture et al. 2012 ) using Fast SYBR®Green master mix (Applied Biosystems, Foster City, CA). .. A total of 5 × 10 4 mouse peritoneal macrophages were plated into 96-well tissue culture plates, incubated overnight, and treated with peptides with or without TLR2 agonist for 2 or 5 h, followed by 3 h incubation with 0.5 mg ml −1 MTT (3-(4, 5-Dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) (Sigma-Aldrich).

Article Title: Caffeic Acid and Metformin Inhibit Invasive Phenotype Induced by TGF-β1 in C-4I and HTB-35/SiHa Human Cervical Squamous Carcinoma Cells by Acting on Different Molecular Targets
Article Snippet: .. Total RNA was isolated from cells using the RNeasy Mini kit (Qiagen, Hilden, Germany) and the quantity of total RNA was measured using a NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). cDNA synthesis was performed using oligo (dT) 15 primer and GoScript Transcriptase according to manufacturer’s instructions (Promega GmbH, Mannheim, Germany). .. The PCR mixture contained: 1.5 uL of cDNA, 1× PCR buffer (Sigma Aldrich) 2.5 mM Magnesium Chloride (Sigma Aldrich), 0.2 mM dNTPs (Sigma Aldrich), 0.2 uM of each primer (Sigma Aldrich), and 1.25 U JumpStartTM Taq DNA Polymerase (Sigma Aldrich).

Synthesized:

Article Title: Inhibitors of TLR signaling by targeting TIR domain interfaces
Article Snippet: Protein was quantified using Bio-Rad protein quantification kit. .. Quantitative Real-Time RT-PCR Total RNA was isolated with Nucleospin RNA II kits (Macherey-Nagel, Bethlehem, Pa.), followed by DNase digestion. cDNA was synthesized from 1 μg RNA using Goscript transcriptase (Promega), and subjected to real-time PCR with gene-specific primers for HPRT, TNF-α, IL-1β, IFNβ, and RANTES on H7900 ABI system using Fast SYBR Green master mix (Applied Biosystems, Foster City, Calif.). .. The cell extracts containing equal amount of protein were incubated with 1 μg Ab for 2 h, followed by 4-h incubation with 25 μl protein G agarose beads (Roche); the beads were then washed four times with lysis buffer, boiled in 13 sample loading buffer, and analyzed by immunoblotting.

Article Title: Differential adapter recruitment by TLR2 co-receptors
Article Snippet: Supernatants were spotted into PVDF membrane, followed by immunoblotting with rabbit anti-Antp Ab (Abcam, Cambridge, MA). .. cDNA was synthesized from 1 μg of total RNA isolated with Nucleospin RNA II kits (Macherey-Nagel, Inc., Bethlehem, PA) using Goscript transcriptase (Promega, Madison, WI), and subjected to real-time PCR (RT-PCR) with gene-specific primers for HPRT, TNF-α or IL-6 (Couture et al. 2012 ) using Fast SYBR®Green master mix (Applied Biosystems, Foster City, CA). .. A total of 5 × 10 4 mouse peritoneal macrophages were plated into 96-well tissue culture plates, incubated overnight, and treated with peptides with or without TLR2 agonist for 2 or 5 h, followed by 3 h incubation with 0.5 mg ml −1 MTT (3-(4, 5-Dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) (Sigma-Aldrich).

Real-time Polymerase Chain Reaction:

Article Title: Inhibitors of TLR signaling by targeting TIR domain interfaces
Article Snippet: Protein was quantified using Bio-Rad protein quantification kit. .. Quantitative Real-Time RT-PCR Total RNA was isolated with Nucleospin RNA II kits (Macherey-Nagel, Bethlehem, Pa.), followed by DNase digestion. cDNA was synthesized from 1 μg RNA using Goscript transcriptase (Promega), and subjected to real-time PCR with gene-specific primers for HPRT, TNF-α, IL-1β, IFNβ, and RANTES on H7900 ABI system using Fast SYBR Green master mix (Applied Biosystems, Foster City, Calif.). .. The cell extracts containing equal amount of protein were incubated with 1 μg Ab for 2 h, followed by 4-h incubation with 25 μl protein G agarose beads (Roche); the beads were then washed four times with lysis buffer, boiled in 13 sample loading buffer, and analyzed by immunoblotting.

Article Title: Differential adapter recruitment by TLR2 co-receptors
Article Snippet: Supernatants were spotted into PVDF membrane, followed by immunoblotting with rabbit anti-Antp Ab (Abcam, Cambridge, MA). .. cDNA was synthesized from 1 μg of total RNA isolated with Nucleospin RNA II kits (Macherey-Nagel, Inc., Bethlehem, PA) using Goscript transcriptase (Promega, Madison, WI), and subjected to real-time PCR (RT-PCR) with gene-specific primers for HPRT, TNF-α or IL-6 (Couture et al. 2012 ) using Fast SYBR®Green master mix (Applied Biosystems, Foster City, CA). .. A total of 5 × 10 4 mouse peritoneal macrophages were plated into 96-well tissue culture plates, incubated overnight, and treated with peptides with or without TLR2 agonist for 2 or 5 h, followed by 3 h incubation with 0.5 mg ml −1 MTT (3-(4, 5-Dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) (Sigma-Aldrich).

SYBR Green Assay:

Article Title: Inhibitors of TLR signaling by targeting TIR domain interfaces
Article Snippet: Protein was quantified using Bio-Rad protein quantification kit. .. Quantitative Real-Time RT-PCR Total RNA was isolated with Nucleospin RNA II kits (Macherey-Nagel, Bethlehem, Pa.), followed by DNase digestion. cDNA was synthesized from 1 μg RNA using Goscript transcriptase (Promega), and subjected to real-time PCR with gene-specific primers for HPRT, TNF-α, IL-1β, IFNβ, and RANTES on H7900 ABI system using Fast SYBR Green master mix (Applied Biosystems, Foster City, Calif.). .. The cell extracts containing equal amount of protein were incubated with 1 μg Ab for 2 h, followed by 4-h incubation with 25 μl protein G agarose beads (Roche); the beads were then washed four times with lysis buffer, boiled in 13 sample loading buffer, and analyzed by immunoblotting.

Article Title: Differential adapter recruitment by TLR2 co-receptors
Article Snippet: Supernatants were spotted into PVDF membrane, followed by immunoblotting with rabbit anti-Antp Ab (Abcam, Cambridge, MA). .. cDNA was synthesized from 1 μg of total RNA isolated with Nucleospin RNA II kits (Macherey-Nagel, Inc., Bethlehem, PA) using Goscript transcriptase (Promega, Madison, WI), and subjected to real-time PCR (RT-PCR) with gene-specific primers for HPRT, TNF-α or IL-6 (Couture et al. 2012 ) using Fast SYBR®Green master mix (Applied Biosystems, Foster City, CA). .. A total of 5 × 10 4 mouse peritoneal macrophages were plated into 96-well tissue culture plates, incubated overnight, and treated with peptides with or without TLR2 agonist for 2 or 5 h, followed by 3 h incubation with 0.5 mg ml −1 MTT (3-(4, 5-Dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) (Sigma-Aldrich).

Spectrophotometry:

Article Title: Caffeic Acid and Metformin Inhibit Invasive Phenotype Induced by TGF-β1 in C-4I and HTB-35/SiHa Human Cervical Squamous Carcinoma Cells by Acting on Different Molecular Targets
Article Snippet: .. Total RNA was isolated from cells using the RNeasy Mini kit (Qiagen, Hilden, Germany) and the quantity of total RNA was measured using a NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). cDNA synthesis was performed using oligo (dT) 15 primer and GoScript Transcriptase according to manufacturer’s instructions (Promega GmbH, Mannheim, Germany). .. The PCR mixture contained: 1.5 uL of cDNA, 1× PCR buffer (Sigma Aldrich) 2.5 mM Magnesium Chloride (Sigma Aldrich), 0.2 mM dNTPs (Sigma Aldrich), 0.2 uM of each primer (Sigma Aldrich), and 1.25 U JumpStartTM Taq DNA Polymerase (Sigma Aldrich).

cDNA Synthesis:

Article Title: Caffeic Acid and Metformin Inhibit Invasive Phenotype Induced by TGF-β1 in C-4I and HTB-35/SiHa Human Cervical Squamous Carcinoma Cells by Acting on Different Molecular Targets
Article Snippet: .. Total RNA was isolated from cells using the RNeasy Mini kit (Qiagen, Hilden, Germany) and the quantity of total RNA was measured using a NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). cDNA synthesis was performed using oligo (dT) 15 primer and GoScript Transcriptase according to manufacturer’s instructions (Promega GmbH, Mannheim, Germany). .. The PCR mixture contained: 1.5 uL of cDNA, 1× PCR buffer (Sigma Aldrich) 2.5 mM Magnesium Chloride (Sigma Aldrich), 0.2 mM dNTPs (Sigma Aldrich), 0.2 uM of each primer (Sigma Aldrich), and 1.25 U JumpStartTM Taq DNA Polymerase (Sigma Aldrich).



Similar Products

90
Promega goscript reverse transcriptase
Goscript Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscript+transcriptase/goscripttm+reverse+transcription+system/pmc12269245-32-8-16
Average 90 stars, based on 1 article reviews
goscript reverse transcriptase - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

90
Promega goscript reverse transcriptase mix with oligo(dt) primers
Goscript Reverse Transcriptase Mix With Oligo(dt) Primers, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscript+transcriptase/goscripttm+reverse+transcription+system/pm40632846-269-14-21
Average 90 stars, based on 1 article reviews
goscript reverse transcriptase mix with oligo(dt) primers - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

90
Promega goscript reverse transcriptase system
Goscript Reverse Transcriptase System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscript+transcriptase/goscripttm+reverse+transcription+system/pm40593752-432-1-5
Average 90 stars, based on 1 article reviews
goscript reverse transcriptase system - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

90
Promega goscript reverse transcriptase kit
Goscript Reverse Transcriptase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscript+transcriptase/goscript+reverse+transcriptase+kit/pmc12269486-135-6-10
Average 90 stars, based on 1 article reviews
goscript reverse transcriptase kit - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

Image Search Results